Regulatory

Part:BBa_K2003030:Design

Designed by: Jakob Nyberg   Group: iGEM16_Uppsala   (2016-09-01)


pBad/araC promoter + medium RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We thought the pBad might be tightly regulated.


Source

BBa_I0500 and BBa_B0032

References